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The primary antibodies from Bioss (Beijing, China) included anti-scleraxis (SCX, bs-12364R), anti-tenascin C (TNC, bs-1327R), anti-tenomodulin (TNMD, bs-7525R), anti-collagen type 1 alpha 1 (COLIA1, bs-10423R), anti-integrin 5 (bs-0567R), and anti-integrin 1 (bs-0486R)